Potassium currents of isolated Necturus enterocytes: a whole-cell patch-clamp study.
نویسندگان
چکیده
منابع مشابه
Whole-Cell Patch-Clamp Recordings
The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
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A distinct subpopulation of neurons in the rostral and ventrolateral part of the medulla oblongata (RVL) plays a key role in controlling sympathetic vasomotor tone. To characterize these neurons under conditions in which all cell-to-cell interactions are eliminated, RVL neurons were acutely dissociated from 13- to 19-day old rats. Cells projecting to the upper thoracic segments were retrogradel...
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Patch clamp method developed more than 30 years ago is widely used for investigation of cellular excitability manifested as transmembrane ionic current and/or generation of action potentials. This technique could be applied to measurement of ionic currents flowing through individual (single) ion channels or through the whole assembly of ion channels expressed in the whole cell. Whole cell confi...
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To isolate inward Ca2+ currents in GH3 rat pituitary cells, an inward Na+ current as well as two outward K+ currents, a transient voltage-dependent current (IKV) and a slowly rising Ca2+-activated current (IKCa), must be suppressed. Blockage of these outward currents, usually achieved by replacement of intracellular K+ with Cs+, reveals sustained inward currents. Selective blockage of either K+...
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The patch clamp technique was first used by Neher and Sakmann (1976) to resolve currents through single acetylcholine-activated channels in cell-attached patches of membrane of frog skeletal muscle. The method they used (described by Neher, Sakmann & Steinbach, 1978) and subsequent refinements (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) have led to techniques for high resolution recording ...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1991
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1991.sp018449